ARS | Publication request: The MSC phenotype of cucumber (Cucumis sativus L.) - a method for production of plant mitochondrial mutants

Submitted to: Journal of Applied Genetics
Publication Type: Review Article
Publication Acceptance Date: December 13, 2006
Publication Date: January 13, 2007
Citation: Bartoszewski, G., Havey, M.J., Ziolkowska, A., Malepszy, S. 2007. The MSC phenotype of cucumber (Cucumis sativus L.) - a method for production of plant mitochondrial mutants. Journal of Applied Genetics. 48(1):1-9.

Technical Abstract: The mosaic (MSC) mutants of cucumber (Cucumis sativus L.) appear after passage through cell cultures. The MSC phenotype shows paternal transmission and is associated with mitochondrial DNA rearrangements. This review describes the origins and phenotypes of independently produced MSC mutants of cucumber, including current knowledge about their mitochondrial DNA rearrangements , and similarities of MSC with other plant mitochondrial mutants. Finally we propose that passage of cucumber through cell culture represents a unique and efficient method to generate mitochondrial mutants of a higher plant in a highly homozygous nuclear background.