ARS | Publication request: Supported Phospholipid Membrane Formation as a Function of 1,2-Dimyristoylphosphatidylglycerol

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: December 2, 2006
Publication Date: December 2, 2006
Citation: Evans, K.O., Hawkins, G. 2006. Supported phospholipid membrane formation as a function of 1,2-dimyristoylphosphatidylglycerol [abstract]. p. 34. Available: http://www.q-sense.com/2006_wc_flash_presentations/day_2_pres_06_kervin_evans/day%202%20pres%2006%20kervin%20evans.html.

Technical Abstract: The ability to form a reasonably supported phospholipid membrane (SPB) on a surface is vital to our development of a bioreactor that entails electron transfer proteins immobilized on a thiol-modified surface. In this respect, we will present work where we investigated what the influence of calcium and the anionic lipid dimyristoylphosphatidylglycerol (DMPG) had on supported bilayer formation on various surfaces spanning from silica (where SPB readily form) to bare & thiol-modified gold (underlying surfaces for our bioreactor). Results have shown that typical a) in the presence of calcium, vesicles adsorbed to all surfaces at a decreased rate as DMPG was increased in concentration; complete SPB formation occurred on only silica when DMPG was present up to 20-mole percent; less water was present in vesicles that adsorbed to the thiol-modified gold surface than was present in vesicles that adsorbed to bare gold; b) in the absence of calcium, vesicles adsorbed less and less to bare and modified gold as DMPG was increased in concentration. However, we surprisingly found that SPB slowly formed on silica in the absence of calcium when DMPG was present at 50-mole percent (unlike phosphatidylserine which is also anionic). We believe that this suggests that lipid headgroup shape, as well as charge, will play a role in SPB on surfaces.