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Title: A practical guide to the isolation, analysis and identification of conjugated linoleic acid

Author
item CHRISTIE, WILLIAM - SCOTTISH CROP RES INST
item DOBSON, GARY - SCOTTISH CROP RES INST
item Adlof, Richard

Submitted to: Lipids
Publication Type: Review Article
Publication Acceptance Date: 7/25/2007
Publication Date: 8/21/2007
Citation: Christie, W.W., Dobson, G., Adlof, R.O. 2007. A practical guide to the isolation, analysis and identification of conjugated linoleic acid. Lipids. 42:1073-1084.

Interpretive Summary:

Technical Abstract: Natural and synthetic conjugated linoleic acids (CLA) are reputed to have therapeutic properties that are specific to particular geometrical and positional isomers. Analysis of these has presented unique problems that have brought forward distinctive solutions, especially the use of gas chromatography-mass spectrometry and silver-ion high-performance liquid chromatography. In the analysis of CLA present at low levels in tissue samples, it is sometimes necessary to use concentration methods. In this review, the most useful and practical methods for the isolation and analysis of CLA isomers in tissues and in commercial CLA preparations are described.