Title: ANTIBODY TO A SHORT PEPTIDE SEQUENCE DETECTED SUGARCANE YELLOW LEAF VIRUS ISOLATES FROM SEVERAL SOURCES Authors
|Wang, Ming - HARC|
|Schenk, Susan - HARC|
Submitted to: International Society of Sugar Cane Technologists Pathology Workshop
Publication Type: Abstract Only
Publication Acceptance Date: December 20, 2005
Publication Date: January 20, 2006
Citation: Wang, M., Schenk, S. 2006. Antibody to a short peptide sequence detected Sugarcane Yellow Leaf Virus isolates from several sources. International Society of Sugar Cane Technologists Pathology Workshop, Programme and Abstracts, p. 19. Technical Abstract: Sugarcane Yellow Leaf Virus (SCYLV) infects many sugarcane cultivars in sugarcane-growing areas around the world. Infected plants are often symptomless and diagnosis depends on PCR analysis or on one of several immunology techniques which require the use of a specific antibody. Although it has been done successfully, purification of SCYLV from infected plants is difficult because the virus is restricted to phloem cells and exists in low titre. Slight amounts of sugarcane proteins are likely to remain which may interfere with specificity of an antibody. Therefore, a project was undertaken that made use of published information on other Luteovirus coat protein structures, estimates of possible antigenic epitopes, sequencing of the capsid protein gene from a Hawaiian isolate and testing of antibodies to them for detection and specificity to SCYLV. The result was the production of one antibody that had the desired activity and specificity to be used for diagnosis of various isolates of SCYLV without interfering reactions to sugarcane tissue or to other related Luteoviruses or Poleroviruses.