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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Research » Research » Publications at this Location » Publication #195271
Title: GENETIC MAPPING AND QTL ANALYSIS OF PYREONPHORA TERES F. TERES GENES CONFERRING AVIRULENCE ON BARLEY.

Author
item LAI, ZHIBING - NORTH DAKOTA STATE UNIV
item Faris, Justin
item Weiland, John
item STEFFENSON, BRIAN - UNIV OF MINNESOTA
item Friesen, Timothy

Submitted to: Fungal Genetics and Biology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/29/2006
Publication Date: 1/16/2007
Citation: Lai, Z., Faris, J.D., Weiland, J.J., Steffenson, B.J., Friesen, T.L. 2007. Genetic mapping of Pyreonphora teres f. teres genes conferring avirulence on barley. Fungal Genetics and Biology. 44:323-329.

Interpretive Summary: Net blotch is one of the most widely distributed diseases of barley, and is caused by a fungus designated Pyrenophora teres f. teres. A population of this fungus was used to evaluate the genetics of avirulence associated with barley lines Canadian Lake Shore (CLS), Tifang, and Prato. Parental isolates (those fungal isolates that were crossed to make the population) caused different reactions on the three barley lines and therefore we hypothesized that each of the parents contained different genes associated with avirulence. A molecular map was constructed to identify genes associated with disease. A single gene (AvrHar) conferred avirulence to Tifang and CLS and accounted for 84 and 85% of the disease, respectively. Avirulence on Prato was conferred by two genes accounting for 25% (AvrPra1) and 32% (AvrPra2) of the disease. AvrPra2 was at the same chromosomal location as AvrHar, but was contributed by the opposite parent. This work provides the foundation for the characterization of these genes.

Technical Abstract: A Pyrenophora teres f. teres cross between isolates 0-1 and 15A was used to evaluate the genetics of avirulence associated with barley lines Canadian Lake Shore (CLS), Tifang, and Prato. 15A is avirulent on Tifang and CLS, but virulent on Prato. Conversely, 0-1 is avirulent on Prato, but virulent on Tifang and CLS. Avirulence conferred by 15A on Tifang and CLS segregated 1:1, whereas avirulence on Prato segregated 3:1. A linkage map was constructed to identify QTLs associated with the disease phenotype. A single locus derived from 15A (AvrHar) conferred avirulence to Tifang and CLS and accounted for 84 and 85% of the phenotypic variation, respectively. Avirulence on Prato was conferred by 0-1 at two redundant loci accounting for 25% (AvrPra1) and 32% (AvrPra2) of the disease variation. AvrPra2 co-segregated with AvrHar, but was contributed by 0-1. This work provides the foundation for the isolation of these avirulence genes.