|Kuster, Helge - BIELEFELD, GERMANY|
|Becker, Anke - BIELEFELD, GERMANY|
|Tesfaye, Mesfin - UNIVERSITY OF MINNESOTA|
Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: July 1, 2006
Publication Date: December 8, 2006
Citation: Kuster, H., Becker, A., Samac, D.A., Tesfaye, M. 2006. Transcriptomics. In: The Medicago truncatula Handbook. The Samuel Roberts Noble Foundation, Ardmore, OK. Available: http://www.noble.org/MedicagoHandbook/. Technical Abstract: This chapter presents the current status of microarray and GeneChip tools in the model legume Medicago truncatula. After a short introduction into the major tools available, detailed protocols that can be used to obtain expression profiles from cDNA macroarrays, 70mer long oligonucleotide microarrays and Affymetrix Medicago GeneChips are listed. These protocols are successfully applied in the author's laboratories to obtain expression profiles from a range of Medicago truncatula tissues and growth conditions. Depending on the equipment of expression profiling facilities, modifications might be required to suit individual demands. Therefore, please use these protocols as a starting point and adjust them after consulting experienced personnel at your local facilities.