|Xu, Fangxiu - UNIVERSITY OF ILLINOIS|
|Leakey, Andrew D - UNIVERISTY OF ILLINOIS|
|Gillespie, Kwlly - UNIVERSITY OF ILLINOIS|
Submitted to: American Society of Plant Biologists
Publication Type: Abstract Only
Publication Acceptance Date: November 17, 2006
Publication Date: November 17, 2006
Citation: Xu, F., Leakey, A.B., Gillespie, K.M., Ainsworth, E.A., Ort, D.R. 2006. Gene expression profiles of soybean from face exposure to elevated tropospheric [O3] and [CO2] [abstract]. American Society of Plant Biologists. Paper No. PO2015. http://abstracts.aspb.org/pb2006/public/PO2/PO2015.html. Technical Abstract: Ozone (O3) has become an important pollutant in the troposphere in many agricultural regions and is predicted to become worse. It is well established that prolonged exposure to [O3] can decrease leaf photosynthesis and accelerate senescence once it enters the plant through stomata. Elevated [CO2] is known to decrease stomatal conductance, thus restricting the entry of [O3] into the leaf. We are investigating the effect of elevated [O3] and the interaction between elevated [O3] and [CO2] on global gene expression patterns of soybean grown in the field at the SOYbean Free-Air Concentration Enrichment (SoyFACE) facility at the University of Illinois. Soybean was grown in four plots at ambient [CO2] (~380 ppm), four plots at elevated [O3] (1.25 times the current annual exposure), four plots at elevated [CO2] (~550 ppm) and four plots with combined elevated [CO2] and elevated [O3], from sowing until harvest. In 2005 RNA was extracted from the youngest, fully expanded soybean leaves. Microarray analysis of the gene expression profile was conducted using 40 Affymetrix Soybean Genome Array GeneChips across 3 reproductive growth stages. Changes in gene expression will be discussed in relation to photosynthetic physiology, leaf biochemistry and water relations. This will provide a unique characterization of crop responses to growth at elevated [CO2] and [O3] under field conditions.