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United States Department of Agriculture

Agricultural Research Service

Research Project: PATHOGEN REDUCTION AND OPTIMIZATION OF WATER USAGE IN POULTRY PROCESSING OPERATIONS

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Title: Comparison of Growth of Campylobacteriaceae on Media Supplemented with Organic Acids and on Commerically Available Media

Author
item Hinton, Jr, Arthur

Submitted to: International Journal of Poultry Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: February 21, 2006
Publication Date: March 21, 2006
Citation: Hinton Jr, A. 2006. Comparison of growth of campylobacteriaceae on media supplemented with organic acids and on commerically available media. International Journal of Poultry Science. 5(2):99-103.

Interpretive Summary: Experiments were conducted to compare the ability of bacteriological media to support growth of campylobacter. Growth of the bacteria in a newly developed medium and in commercial media was measured. The new, liquid medium was composed of proteins and a mixture of several organic acids. Growth of the bacteria in liquid media was compared in the new medium and in Brucella broth, Mueller-Hinton broth, and Fluid Thioglycollate medium. Eleven of 16 of the bacteria grew better in the new media than in commercially available media. There was no difference in growth of the other 5 bacteria in the new media or in the commercial liquid media. Growth of the bacteria was also compared on solid media. The new solid media was prepared by using agar to solidify the liquid media. The number of bacteria recovered on the new solid media was compared to the number of bacteria recovered on commercial blood agar medium. Results indicated that there was no difference in the number of bacteria recovered on the new solid medium and on commercial blood agar medium. Experimental findings showed that the newly developed media may be used as alternatives to some currently available commercial media for growing and maintaining campylobacter bacteria.

Technical Abstract: Experiments were conducted to compare the ability of a medium supplemented with organic acids to the ability of commercially available, non-selective media to support growth of Campylobacter spp. and Arcobacter butzleri. Liquid medium was composed of yeast extract-peptone basal broth (BB) supplemented with an organic acid (OA) mixture of fumaric, lactic, malic, and succinic acids (BB + OA). Growth of cultures in BB and in BB + OA was compared to growth in Brucella broth, Mueller-Hinton broth, and Fluid Thioglycollate medium. Growth of A. butzleri and 10 of 15 Campylobacter isolates was significantly (P < 0.05) greater in BB + OA than in other broth media, while there was no significant difference in growth of the other 5 Campylobacter isolates in BB, BB + OA, Brucella broth, Mueller-Hinton broth, or Fluid Thioglycollate medium. Growth was also compared on agar media composed of BB + OA with Bacto agar and hemin (BA-hemin + OA) and on Remel Blood Agar. Results indicated that there was no significant difference in the number of cfu’s recovered on BA-hemin + OA and on blood agar. Findings indicate that media supplemented with organic acids may be used as alternatives to some currently available commercial media for growing and maintaining cultures of Campylobacteriaceae.

Last Modified: 10/22/2014
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