ARS | Publication request: USE OF MICROSCOPY TO QUANTIFY LIPID LOCATIONS ON/IN RICE KERNELS

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: May 12, 2006
Publication Date: June 13, 2006
Citation: Wood, D.F. Use of microscopy to quantify lipid locations on/in rice kernels. Invited presentation at the University of Arkansas Industry Alliance Meeting, May 25-26, 2005, Fayetteville, Arkansas, Paper No, 7..

Technical Abstract: The objective of this research is to use microscopy methods to determine whether chemical methods used to describe the degree of milling (DOM) represent “surface” or “total” lipids. This presentation serves to update the progress of our research using microscopy to our collaborators at the University of Arkansas, other ARS agencies doing rice research and the industry partners. Lipid bodies were located on and in the rice kernel using fluorescence microscopy both before and after various degrees of milling as well as after the surface extraction of lipid. Lipids were located using nile blue A, the active component of which is Nile Red, which fluoresces most intensely with neutral lipids. Other microscopy methods will be used to describe and correlate surface (scanning electron microscopy) and interior (light microscopy) changes resulting from milling and extraction can be described.