IDENTIFICATION AND CHARACTERIZATION OF GENES AFFECTING COOL AND COLD WATER AQUACULTURE PRODUCTION
Location: Cool and Cold Water Aquaculture Research
Title: PCR PRIMERS FOR TRINUCLEOTIDE AND TETRANUCLEOTIDE MICROSATELLITES IN GREATER AMBERJACK, SERIOLA DUMERILI
| Renshaw, Mark - TEXAS A&M UNIVERSTIY |
| Patton, John - TEXAS A&M UNIVERSTIY |
| Gold, John - TEXAS A&M UNIVERSTIY |
Submitted to: Molecular Ecology Notes
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: May 24, 2006
Publication Date: December 1, 2006
Citation: Renshaw, M.A., Patton, J.C., Rexroad III, C.E., Gold, J.R. 2006. Pcr primers for trinucleotide and tetranucleotide microsatellites in greater amberjack, seriola dumerili. Molecular Ecology Notes 6: 1162-1164.
Interpretive Summary: Greater amberjack, Seriola dumerili, is an economically important, reef-associated carangid fish with a circumglobal distribution in sub-tropical and temperate waters. In U.S. waters, increased commercial and recreational fishing pressure on this species has led to establishment of fishery limitations along the Atlantic coast and an assessment of the species as 'overfished' in the northern Gulf of Mexico. Additionally, there is worldwide interest in commercial aquaculture of greater amberjack due to its relatively rapid growth rate and excellent market potential. We report development of 18 genetic markers for (i) providing population-genetic data to assist with management of 'wild' stocks, and (ii) applications in aquaculture investigations, including assessment of genetic variability and inbreeding, parentage assignment, and quantitative trait loci (QTL) identification.
Eighteen nuclear-encoded microsatellites from a genomic DNA library of greater amberjack, Seriola dumerili, were isolated and characterized. The microsatellites include 13 perfect (five tetranucleotide and eight trinucleotide) and five imperfect (three tetranucleotide, one trinucleotide, and one combination dinucleotide/trinucleotide) repeat motifs. The number of alleles at the 18 microsatellites among a sample of 29 fish ranged from two to 20; gene diversity (expected heterozygosity) ranged from 0.068 to 0.950, while observed heterozygosity ranged from 0.069 to 0.966. Following Bonferroni correction, genotypes at all eighteen microsatellites fit expectations of Hardy-Weinberg equilibrium and all pairwise comparisons of microsatellites did not deviate significantly from genotypic equilibrium. Greater amberjack support commercial and recreational fisheries along both the Atlantic and Gulf coasts of the U.S. and represent a species with potential for worldwide aquaculture. The microsatellites developed will be useful for population-genetic studies of 'wild' populations and breeding studies of domesticated populations.