ARS | Publication request: MOLECULAR CHARACTERIZATION OF SEVERAL NEW AND EMERGING POTYVIRUSES OF ORNAMENTAL PLANTS

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: March 30, 2006
Publication Date: June 1, 2006
Citation: Jordan, R.L., Guaragna, M.A. 2006. Molecular characterization of several new and emerging potyviruses of ornamental plants. Phytopathology. 96:S56.

Technical Abstract: Diseases caused by viruses continue to seriously affect the production and quality of ornamental plants. Growers have reported problems with previously unreported viruses in several economically important ornamental crop species exhibiting virus-like symptoms. Total RNA was isolated from ornamental plants exhibiting mosaic, ringspot, or flower break symptoms that were ELISA-positive using our potyviral broad-spectrum monoclonal antibody PTY-1. Potyvirus 'generic' primers were used in RT-PCR to amplify 3' terminal ca. 1600bp fragments (includes coat protein gene and 3' NCR). PTY-1 immunocaptured (IC) virions were also used in IC-RT-PCR reactions. The resultant PCR amplicons were cloned, sequenced and compared to potyvirus sequences in the international databases. Serological and sequence analysis revealed: the identification of new potyviruses Impatiens flower break virus (in New Guinea Impatiens), Tricyrtis virus Y (in toad lily Tricyrtis formosana), Spiranthes mosaic virus 2 and Spiranthes mosaic virus 3 (in the terrestrial orchid Spiranthes cernua), and, Omphalodes virus Y (in Navelwort Omphalodes); the first reports of Dasheen mosaic virus in Spiranthes cernua and Bean yellow mosaic virus (BYMV) in Verbena; and, previously identified reoccurring potyviruses infecting Euphorbia (Euphorbia ringspot virus), Osteospermum (Lettuce mosaic virus), and Schizostylis (BYMV).