|Qin, Feng - SHANHAI INST OF DRUG CONT|
Submitted to: Meeting Abstract
Publication Type: Proceedings
Publication Acceptance Date: July 10, 2006
Publication Date: October 1, 2006
Citation: Chen, G., Qin, F. 2006. Analysis of oxytetracycline in surface water using a portable analyzer. Proceedings SPIE 6378, 6378W. Technical Abstract: A huge amount of oxytetracycline (OTC) is used in aquaculture worldwide. Since it is poorly absorbed by the gastrointestinal system and has a long lifetime, it is an important task to monitor its distribution and fate in the marine environment and its impact on human and the biosphere. A portable LED-based analyzer was prototyped employing europium-sensitized luminescence to perform field analysis of OTC in water, sediment, and other marine environmental matrices. In this study a simple and sensitive method for OTC analysis in surface water is developed using this instrument. OTC is first extracted and enriched from water onto 17×15 mm C18 sorbent strips under 1-hr constant stirring. The strips are then dipped into a Eu-containing reagent solution for 2 min. Next the dried strips are desiccated and exposed to a pulsed 385-nm UV beam. The time-resolved luminescence is directly measured on the sorbent surface. A 0-100 ppb linear dynamic range (LDR) (r2 = 0.999) and a 0.13-ppb limit of detection (LOD) are achieved with a typical 5% relative standard deviation (RSD). Based on the experimental results, this portable instrument and the assay method can be applied to aquacultural system monitoring, and to environmental analysis in general.