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United States Department of Agriculture

Agricultural Research Service

Title: Detection of Mattesia Oryzaephili in Cryptolestes Ferrugineus (Coleoptera: Laemophoeidae) Laboratory Colonies with An Enzyme-Linked Immunosorbent Assay

item Lord, Jeffrey

Submitted to: Journal of Invertebrate Pathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: July 31, 2006
Publication Date: October 1, 2006
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Citation: Lord, J.C. 2006. Detection of Mattesia oryzaephili (Neogregarinorida: Lipotrophidae) in grain beetle laboratory colonies with an enzyme-linked immunosorbent assay. Journal of Invertebrate Pathology 94: 74-76.

Interpretive Summary: The impact of naturally occurring insect pathogens on population of stored-grain pests is not known. Pathogens are often present but not detected in laboratory insect colonies and may interfere with experimental work. Researchers have not had tools that can easily detect infections in large insect samples. A serological test (enzyme-linked immunosorbent assay) with highly specific monoclonal antibodies was developed for the detection of Mattesia oryzaephili, a pathogen of grain beetles. Seven rusty grain beetle laboratory cultures from the United States, Canada, and Australia were tested with the assay. All of the cultures were infected with the pathogen. This assay will make possible epizootiological studies to assess the impact of M. oryzaephili on pest populations and to determine the potential to exploit the pathogen by conservation or introduction where it does not occur. It will also allow scientists to be aware of problems in their cultures that hamper their work.

Technical Abstract: An indirect sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of the neogregarine Mattesia oryzaephili was developed with monoclonal antibodies. It was used to screen laboratory colonies of the rusty grain beetle Cryptolestes ferrugineus from the United States, Canada, and Australia. All seven colonies had larvae that tested positive with the percent of positives ranging from 0.54 to 83.9. This assay will make possible epizootiological studies to assess the impact of M. oryzaephili on pest populations.

Last Modified: 5/5/2015
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