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United States Department of Agriculture

Agricultural Research Service

Research Project: NEW APPROACHES FOR INSECT PEST MANAGEMENT IN VEGETABLE CROPS

Location: Vegetable Research

Title: Resistance of Sweetpotato Genotypes to Adult Diabrotica Beetles

Authors
item Jackson, David
item Bohac, Janice

Submitted to: Journal of Economic Entomology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: September 6, 2006
Publication Date: April 16, 2007
Citation: Jackson, D.M., Bohac, J. 2007. Resistance of Sweetpotato Genotypes to Adult Diabrotica Beetles. Journal of Economic Entomology. 100:566-572.

Interpretive Summary: Production of sweetpotatoes is severely limited by several insect pests, including cucumber beetles, and new pest management approaches for this crop are needed. A host plant resistance research program typically depends on reliable bioassay procedures to streamline evaluation of germplasm. Thus, a bioassay technique was developed for adults of banded and spotted cucumber beetles. For this bioassay, a piece of sweetpotato skin was embedded in plaster and a single adult was placed on it. Plugs were changed as needed and adult longevity was measured. Adult longevity ranged from approximately 7-8 days for starved individuals to over 200 days for beetles fed a dry artificial diet. Longevity of adults fed sweetpotato skins ranged from 10 days for the most resistant genotypes to over 100 days for a susceptible variety. This bioassay was consistent with field results, indicating that this technique could be useful for evaluating pest resistance in sweetpotato to cucumber beetles.

Technical Abstract: Production of sweetpotatoes is severely limited by several insect pests, including Diabrotica spp., and new pest management approaches for this crop are needed. A host plant resistance research program typically depends on reliable bioassay procedures to streamline evaluation of germplasm. Thus, a bioassay technique was developed for adults of two cucumber beetle species (Diabrotica balteata and D. undecimpunctata). For this bioassay, a piece of sweetpotato peel (periderm & cortex with stele removed) was embedded periderm-side up in plaster in a Petri dish, and a single adult was placed on it. Plugs were changed as needed and adult longevity was measured. Adult longevity ranged from approximately 7-8 days for starved individuals to over 200 days for beetles fed a dry artificial diet. Longevity of adults fed sweetpotato peels ranged from 10 days for the most resistant genotypes to over 100 days for SC1149-19, the susceptible control genotype. This bioassay was consistent with field results, indicating that this technique could be useful for evaluating pest resistance in sweetpotato genotypes for Diabrotica and other insect species.

Last Modified: 9/1/2014
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