Technical Abstract: Blueberry fruit produce antimicrobial volatiles including trans-2-hexenal that may confer resistance to anthracnose fruit rot, an important postharvest disease caused by Colletotrichum acutatum. To test the hypothesis that aromatic volatiles in blueberry fruit may be associated with postharvest fruit rot resistance, we compared volatiles emitted from intact fruit and fruit extracts of C. acutatum-inoculated and non-inoculated blueberry fruit stored 0 to 6 days from cultivars having a wide range of resistance to anthracnose. Antimicrobial volatiles detected included the aldehydes, trans-2-hexenal and hexanal; the monoterpenes, limonene, linalool, 8-hydroxylinalool, ''terpineol, and terpinyl acetate; and the sesquiterpenes, cadinene, caryophyllene and '-farnesene. Volatile emissions from intact fruit and extracts varied amongst the cultivars, with the early ripening cultivars generally showing higher volatile emissions than later ripening cultivars. While trans-2-hexenal, hexanal in extracts, terpinyl acetate, and cadinene emission increased in most cultivars during storage, quantitative changes were not correlated to exposure to anthracnose infection prior to storage. The results suggest that antimicrobial aldehydes and terpenes emitted from fully-ripe blueberry fruit and extracts are not inducible by C. acutatum infection and do not contribute to disease resistance against anthracnose fruit rot.