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United States Department of Agriculture

Agricultural Research Service

Title: Comparative Evaluation of An Automated Ribotyping System Versus Pulsed-Field Gel Electrophoresis for Epidemiology Typing of Veterinary Isolates of Salmonella Enterica Serotype Typhimurium Dt104

item Wallace, Frederick
item Cray, Paula

Submitted to: American Society for Microbiology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: April 21, 2000
Publication Date: May 21, 2000
Citation: Wallace, F.M., Cray, P.J. 2000. Comparative evaluation of an automated ribotyping system versus pulsed-field gel electrophoresis for epidemiology typing of veterinary isolates of salmonella enterica serotype typhimurium dt104. American Society for Microbiology Meeting. Session 193/Z. Abstract Z-26. P. 690.

Technical Abstract: Salmonella infections continue to be a problem in industrialized countries. The strain of Salmonella enterica serotype typhimurium known as definitive phage type 104 (DT104) emerged as a major pathogen in Europe and has since isolated in the U.S. DT104 is of major concern as it is usually resistant to five antimicrobials (ampicillin, chloramphenicol, streptomycin, sulfonamides, and tetracycline) with additional resistance to trimethoprim and ciprofloxacin observed in some isolates. Ribotyping and macrorestriction analysis of chromosomal DNA using pulsed-field gel electrophoresis (PFGE) are among the more useful molecular epidemiologic typing methods in use, with PFGE being the current method of choice for typing strains of Salmonella. In this study we compared PFGE and a commercial ribotyping system (Riboprinter, Qualicon, Inc.) for typing 60 Salmonella DT104 isolates of veterinary origin. Preliminary PFGE typing of the isolates using XbaI indicated resolution into only 3 groups. Automated ribotype analysis of all 60 isolates with EcoRI yielded a single ribotype, supporting the ultimate clonality of DT104 isolates. In other ribotyping studies discrimination between strains of Salmonella typhimurium isolates has been achieved using restriction enzymes such as PvuII and PstI. Preliminary results suggest that PVU II will further subdivide DT104 into more discriminate types which may provide additional information in epidemiologic studies.

Last Modified: 4/19/2015
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