Technical Abstract: Solanum triflorum Nutt. (cutleaf nightshade) poisoning has been associated with gastroenteritis, but poisoned horses have severe salivation, frequent urination, diarrhea and colic. The purpose of this study was to develop a small animal model of poisoning and if possible, identify the neurotoxin. Thirty Syrian hamsters were randomly divided into 5 groups and dosed by gavage with 0, 50, 100, 150 and 200 mg of plant suspended in 2 ml of water four times a day for 8 days. After dosing the animals were euthanized and samples were collected for biochemical and histologic studies. None of the treated hamsters developed cholinergic related clinical signs and there were no significant differences in serum electrolyte or biochemical between the groups. After several days of dosing, the high dose hamsters developed diarrhea and small labial ulcerations. Segmental portions of intestine and stomach were dilated with gas and mucoid exudates in all of the high dose animals. The adjacent mucosa was red and edematous. Histologically these animals had focally extensive hemorrhagic and necrotizing gastroenteritis. These findings suggest that in rodents, cutleaf nightshade toxicity is due to the direct toxic effects of Solanum glycoalkaloids. Additional studies are needed to determine if cutleaf nightshade cholinergic toxins only affect horses or if they are specific to plant phenotype.