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ARS Home » Pacific West Area » Parlier, California » San Joaquin Valley Agricultural Sciences Center » Crop Diseases, Pests and Genetics Research » Research » Publications at this Location » Publication #186594
Title: COMPARATIVE PROTEOMIC ANALYSIS OF STEM TISSUE AND XYLEM SAP FROM PIERCE'S DISEASE RESISTANT AND SUSCEPTIBLE GRAPEVINES

Author
item Lin, Hong
item Fritschi, Felix
item Yang, Litao
item WALKER, ANDREW - UNIV OF CALIF-DAVIS

Submitted to: CDFA Pierce's Disease Control Program Research Symposium
Publication Type: Proceedings
Publication Acceptance Date: 10/1/2005
Publication Date: 12/5/2005
Citation: Lin, H., Fritschi, F.B., Yang, L., Walker, A. 2005. Comparative Proteomic Analysis of Stem Tissue and Xylem Sap from Pierce's Disease Resistant and Susceptible Grapevines. Proceedings of the 2005 Pierce's Disease Research Symposium. p. 47-49.

Interpretive Summary: Protein profiles in xylem sap and stem tissue of highly tolerant and susceptible grape genotypes were determined. Ten sequential samplings of stem tissues have been made from infected and noninfected grapevines 1-12 weeks post inoculation. Protein extraction and analyses have recently been initiated. Plants for xylem sap extraction are currently being grown in the greenhouse. Xylem sap will be extracted multiple times post inoculation from Xf and water-inoculated plants. Differentially expressed proteins in both stem tissue and xylem sap will be identified. Results obtained will deepen our understanding of host-pathogen interactions for managing PD.

Technical Abstract: Both xylem sap and stem tissue are key components in the Xylella fastidiosa-grapevine interaction. In this research we investigate protein expression in xylem sap and stem tissue of highly tolerant and susceptible grape genotypes. Ten sequential samplings of stem tissues have been conducted from infected and noninfected grapevines ranging from 1 day to 12 weeks post inoculation. Protein extraction and analyses on these tissues has recently been initiated. Plants for xylem sap extraction are currently being grown in the greenhouse. Xylem sap will be extracted multiple times post inoculation from Xf and water-inoculated plants. Differentially expressed proteins in both stem tissue and xylem sap will be identified and investigated in more detail in the coming months. Results obtained will deepen our understanding of host-pathogen interactions, a key component in fighting PD.