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United States Department of Agriculture

Agricultural Research Service

Research Project: IDENTIFICATION AND CHARACTERIZATION OF GENES AFFECTING COOL AND COLD WATER AQUACULTURE PRODUCTION

Location: Cool and Cold Water Aquaculture Research

Title: Microsatellite Markers for Population Genetics and Genome Mapping in Striped Bass

Authors
item Rexroad, Caird
item Coulibaly, Issa - WEST VIRGINIA UNIVERSTIY
item Couch, Charlene - NORTH CAROLINA STATE UNIV
item Garber, Amber - NORTH CAROLINA STATE UNI
item Westerman, Mark - KENT SEATECH CORPORATION
item Sullivan, Craig - NORTH CAROLINA STATE UNIV

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: December 15, 2005
Publication Date: December 15, 2005
Citation: Rexroad III, C.E., Coulibaly, I., Couch, C., Garber, A., Westerman, M., Sullivan, C. 2005. Microsatellite markers for population genetics and genome mapping in striped bass. Plant and Animal Genome Conference. Abstract ID P638, page 261.

Technical Abstract: Although the commercial aquaculture of hybrid striped bass ranks fourth among finfish in the US in economic impact, the industry still relies heavily on wild-caught stocks for fingerling production. The construction of genetic markers for Morone species will facilitate the development of domesticated broodstock through selective breeding. To this end, we developed 345 microsatellite markers for population genetic studies and genome mapping. Microsatellite repeat-enriched libraries (AAG, ATG, TACA, TAGA) were used to identify candidate sequences for marker development. A subset of 72 markers was selected for characterization by genotyping across a panel of fish from diverse origins. Fifty-two markers were amplified successfully and found to be polymorphic, with 2-18 alleles and PIC values ranging from .2 to .87. Five markers were observed to amplify multiple loci as would be observed as a result of a genome duplication event. One of these markers had sufficient distance between allele sizes such that it was analyzed as two markers.

Last Modified: 7/25/2014
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