Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: October 7, 2005
Publication Date: January 5, 2006
Citation: Weiland, J.J., Mcgrath, J.M. 2006. An EST database of the sugarbeet pathogen, Aphanomyces Cochlioides [abstract]. Plant & Animal Genome XIV Conference. Abstract No. W181:48. Technical Abstract: Seedling black root and adult root rot caused by the oomycete Aphanomyces cochlioides are perennial problems to sugarbeet production in warm, wet environments. Although genetic resistance in sugarbeet can be used to ward off mild infections of adult plants by the organism, heavy disease pressure cannot be tolerated; seedlings can only be protected by a single, registered fungicide. An expressed sequence tag (EST) database was created to examine the interaction of A. cochlioides with the sugarbeet host. A. cochlioides growing in nutritionally rich artificial medium, in nutritionally lean artificial medium, and invading seedlings of a susceptible sugarbeet hybrid variety was used to produce cDNA libraries. One thousand five hundred single-pass sequence reads were performed on clones from each library which were used for database construction. Organization of the database will permit observation of gene expression differences between the three growth states of A. cochlioides. The database is being integrated into the larger Oomycete Genomics Database at the National Center for Genome Research and is freely available to the research community. Inclusion of an Aphanomyces into this umbrella database that includes Saprolegnia and Phytophthora provides a valuable resource for comparative genomics.