Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: July 13, 2005
Publication Date: July 13, 2005
Citation: Palti, Y., Rodriguez, M.F., Gahr, S.A., Rexroad Iii, C.E., Hansen, J.D. 2005. Genomic sequence and annotation of a region that harbors major histocompatibility genes in rainbow trout. Meeting Abstract. The 3rd International Symposium of Genetics of Animal Health, July 13-15, 2005 held in Ames, Iowa, USA, Meeting Book of Abstracts p. 124. Technical Abstract: We have previously shown that the rainbow trout genome contains at least 4 unlinked regions of major histocompatibility (MH) genes. One of the regions which we previously dubbed the extended MH class II region is located on chromosome arm 3p and harbors the TAP1 (aka ABCB2) gene. TAP1 is one of the two subunits of the transporter associated with antigen processing (TAP), which delivers antigenic peptides into the endoplasmic reticulum for subsequent presentation by MHC class I molecules. In the current study, we developed a BAC contig for the TAP1 region which spans approximately 300 kbp. We have sequenced three of the overlapping BACs to identify and characterize immune response genes and to enable identification of MH haplotypes in rainbow trout. The region contain ORFs with high sequence similarity to genes located in the human class II region such as TAP1 and BRD2, extended class II (RGL2, PHF1), class III (PBX2) and another gene (DAB2ip) which is not located on the mammalian MHC region, but may have functions related to the immune response. The genomic organization and gene content is compared to MH regions in Human, Tetraodon nigroviridis (a puffer fish) and Zebrafish.