Technical Abstract: Red-fleshed watermelon contains lycopene, a carotenoid with antioxidant properties. Identification of germplasm with high amounts of lycopene may help plant breeders and medical researchers develop new uses for natural sources of lycopene. This study was conducted to evaluate lycopene content in commercial cultivars of seeded and seedless red-fleshed watermelons over a five-year period. Spectrophotometric and scanning colorimetric assays of total lycopene were used to separate 65 watermelon cultivars into average (<50 mg/kg fw), high (50-70), very high (70-90), and exceptional (>90) lycopene content categories. Seedless types were usually in the high and very high ranges. Seeded cultivars that were open-pollinated and light red in color (Black Diamond, Calhoun Gray, Crimson Sweet) had an average lycopene concentration, most hybrids had a high concentration, and a few were in the very high range. Carotenoid profiles of watermelons indicated that trans- lycopene was the predominant carotenoid (84-97%) and cultivars differed in the relative amounts of cis-lycopene, beta-carotene, and phytofluene. All carotenoids were correlated to each other. Quadratic relationships were seen between phytofluene and total lycopene and betciween phytofluene and total carotenoids. Phytofluene and beta-carotene had a strong linear relationship. These results demonstrate that red-fleshed watermelon genotypes have the same carotenoid pattern, but relative amounts of carotenoids, especially lycopene, can vary extensively.