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ARS Home » Plains Area » Lincoln, Nebraska » Agroecosystem Management Research » Research » Publications at this Location » Publication #184717
Title: INACTIVATION OF ENTERIC ADENOVIRUS AND FELINE CALICIVIRUS BY OZONE

Author
item Thurston Enriquez, Jeanette
item HAAS, CHARLES - DREXEL UNIV
item JACANGELO, JOSEPH - MONTGOMERY,WATSON HARZA
item GERBA, CHARLES - U OF ARIZONA

Submitted to: Water Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/17/2005
Publication Date: 10/1/2005
Citation: Thurston Enriquez, J.A., Haas, C., Jacangelo, J., Gerba, C. 2005. Inactivation of enteric adenovirus and feline calicivirus by ozone. Water Research. 39(15):3650-3656.

Interpretive Summary: Ozone is an alternative to chlorine for disinfection of drinking water. While studies have shown that ozone is effective at the reduction of several pathogens in water, limited information is available concerning the reduction of emerging viral pathogens. Caliciviruses and enteric adenoviruses are important causes of viral gastroenteritis. Ozone inactivation experiments were conducted with enteric adenovirus type 40 (AD40) and feline calicivirus (FCV). FCV served as a surrogate for human caliciviruses. Experiments were carried out in buffered disinfectant demand free water at pH 7 and 5oC. Under the conditions evaluated by this study, very low levels of ozone in water readily inactivate AD40 and FCV.

Technical Abstract: Little information is available regarding the effectiveness of ozone on the inactivation of caliciviruses and enteric adenoviruses. Inactivation experiments were conducted with feline calicivirus (FCV), closely related to the human caliciviruses based on nucleic acid organization and capsid architecture, and adenovirus type 40 (AD40). Experiments were carried out in buffered disinfectant demand free water at pH 7 and 5 oC. Ct values, concentration of ozone multiplied by contact time with virus, were determined from application of the Efficiency Factor Hom (EFH) Model. Ct values for 4-log (99.99%) ozone inactivation at 5 oC and pH 7 ranged from 0.07 to 0.60 mg/l ´ min for AD40 and <0.01 to 0.03 mg/L ´ min for FCV. Ct values listed in the U.S. Environmental Protection Agency “Guidance Manual for Compliance with Filtration and Disinfection Requirements for Public Water Systems Using Surface Water Sources” were higher than Ct values generated by this study. Very low ozone residuals (<0.01 mg/l) substantially inactivated FCV and AD40 under the studied conditions.