Technical Abstract: Switchgrass is a large, North American, perennial grass that is being evaluated as a potential energy crop. There is a need to assess genetic diversity in stored accessions and in remaining native stands to assist breeding and conservation efforts. Further marker development will also be necessary for linkage mapping. Toward this end, thirytwo switchgrass genic di tri and tetranucleotide repeat microsatellites were identified from ESTsequences. These microsatellites were used to screen individuals from two different named cultivars. The markers displayed a high level of polymorphism consistent with the tetraploid, allogamous behavior of the cultivars tested.