NUTRITIONAL REGULATION OF CELL AND ORGAN GROWTH, DIFFERENTIATION, AND DEVELOPMENT
Location: Children Nutrition Research Center (Houston, Tx)
Title: EFFECT OF ORAL N-CARBAMYLGLUTAMATE (NCG) SUPPLEMENTATION ON GROWTH AND TISSUE PROTEIN SYNTHESIS IN PIGLETS
| Frank, Jason - BAYLOR COLL OF MEDICINE |
| Escobar, Jeffery - BAYLOR COLL OF MEDICINE |
| Suryawan, Agus |
| Liu, Chun - BAYLOR COLL OF MEDICINE |
| Nguyen, Hanh - BAYLOR COLL OF MEDICINE |
| Wu, Guoyao - TEXAS A&M UNIV, ANIM SCI |
Submitted to: Journal of Animal Science Supplement
Publication Type: Abstract Only
Publication Acceptance Date: May 1, 2005
Publication Date: July 24, 2005
Citation: Frank, J., Escobar, J., Suryawan, A., Liu, C., Nguyen, H., Davis, T., Wu, G. 2005. Effect of oral N-carbamylglutamate (NCG) supplementation on growth and tissue protein synthesis in piglets [abstract]. Journal of Animal Science, Proceedings from the 2005 Joint Annual Meeting of the American Dairy Science Association and the American Society of Animal Science. 83(Suppl. 1):abstract 186, p. 118.
Interpretive Summary: Not required for an abstract.
Recent research indicates that oral supplementation of NCG, an analogue of N-acetylglutamate, increases plasma arginine concentrations and growth rate in sow-reared piglets. To investigate the mechanism involved in this response, nursing piglets (n = 14; BW = 2.75 kg) were orally administered 50 mg/kg BW of NCG or saline twice daily from 7 to 14 d of age. After an overnight (12-h) fast, 14-d old piglets were administered saline or NCG at time 0 and 60 min, then received a flooding dose of [3H]phenylalanine in order to measure protein synthesis. At 90 min, the piglets were euthanized and tissue samples were collected. NCG-treated pigs gained more weight during the 7-d trial than control pigs (1.63 +/- 0.07 vs. 1.43 +/- 0.06 kg respectively; P < 0.001). Plasma arginine concentrations were 44% higher in NCG-treated pigs compared to control pigs (P < 0.01). NCG-treated pigs tended to have higher plasma insulin concentrations compared to control pigs (P < 0.06). Plasma glucose concentrations were not different between the treatments (P > 0.73). Fractional protein synthesis rate in longissimus dorsi muscle was 17% higher in NCG-treated pigs compared to controls (6.9 +/- 0.5 vs. 5.9 +/- 0.6 %/d respectively), although this did not reach significance (P < 0.24). Fractional rates of protein synthesis in liver, kidney, and duodenum were not different between treatments (P > 0.49). We postulate that the sensitivity of muscle protein synthesis to NCG treatment in piglets may be reduced in the fasted state. Oral NCG administration increases growth rate of nursing piglets likely by increasing plasma arginine concentrations.