ARS | Publication request: PHYSICAL MAPPING OF FIBER DEVELOPMENT GENES IN COTTON

Submitted to: Plant and Animal Genome
Publication Type: Abstract Only
Publication Acceptance Date: December 1, 2004
Publication Date: January 15, 2005
Citation: Yu, J., Kohel, R.J., Xu, Z., Dong, J., Zhang, H., Stelly, D.M., Zhu, Y., Covaleda, L. 2005. Physical mapping of fiber development genes in cotton [abstract]. Plant and Animal Genome XIII Conference. p. 225.

Technical Abstract: Large numbers of genes are involved in the regulation of fiber development. To understand where these genes located in chromosomes and how they interact is crucial for improving fiber yield and quality. Our goals are to develop integrated physical and transcript maps of fiber initiation, elongation, and cell wall deposition and maturation genes; and to investigate how these genes interact by use of structural and functional genomic tools. Overgo probes were designed from fiber ESTs that were verified by hybridization and expression analyses. Positive clones were fingerprinted and assembled into contigs, and SSR markers from these BACs were used to integrate the contig map to the genetic map and verified by BLAST EST sequences against marker sequence data that are publicly available. In this preliminary report, three contigs of fiber initiation genes were assembled and anchored to chromosome 3, chromosome 13, and linkage group A01, respectively. The three integrated contig maps contained 132 fibers ESTs, 3 BNL SSR markers, 1 CIR SSR marker, and 30 TM1 SSR sequences. Both ends of BAC clones from these contigs were sequenced to identify fiber-gene SSR markers and to verify the integrated physical and transcript maps. The information obtained from the study could help understand genomic distribution and evolutionary events of the fiber genes in Upland cotton.