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United States Department of Agriculture

Agricultural Research Service

Title: Yellow Leaf of Sugarcane Is Caused by at Least Three Different Genotypes of Sugarcane Yellow Leaf Virus, One of Which Predominates on the Island of Reunion

Authors
item Abu Ahmad, Y. - CIRAD-INRA, FRANCE
item Rassaby, L. - CIRAD-INRA, FRANCE
item Royer, M. - CIRAD-INRA, FRANCE
item Borg, Z. - BSES, AUSTRALIA
item Braithwaite, K. - BSES, AUSTRALIA
item Mirkov, E. - TEXAS A&M, TEXAS
item Irey, Michael
item Perrier, X. - CIRAD, FRANCE
item Smith, G. - BSES, AUSTRALIA
item Rott, P. - CIRAD-INRA, FRANCE

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 14, 2005
Publication Date: February 2, 2006
Citation: Abu Ahmad, Y., Rassaby, L., Royer, M., Borg, Z., Braithwaite, K., Mirkov, E., Irey, M.S., Perrier, X., Smith, G., Rott, P. 2006. Yellow leaf of sugarcane is caused by at least three different genotypes of sugarcane yellow leaf virus, one of which predominates on the island of reunion. Archives of Virology. DOI 10.1007/s00705-005-0712-9.

Interpretive Summary: The genetic diversity of Sugarcane yellow leaf virus (SCYLV) was analyzed with 43 virus isolates from Réunion Island and 17 isolates from world wide locations. Phylogenetic analyses performed with obtained sequences and additional published sequences showed that SCYLV isolates were distributed in different phylogenetic groups or belonged to single genotypes. The majority of isolates from Réunion Island were grouped in phylogenetic clusters that did not contain any isolate from other origins. The existence of at least three strains of SCYLV (strain BRA, strain PER and strain REU) was shown by the phylogenetic analysis of the sequence of these isolates and additional published sequences of three SCYLV isolates (GenBank accessions). The biological significance of these strains and the origin of the unique lineage of SCYLV in Réunion Island remain to be determined.

Technical Abstract: The genetic diversity of Sugarcane yellow leaf virus (SCYLV) was analyzed with 43 virus isolates from Réunion Island and 17 isolates from world wide locations. We tried to amplify by RT-PCR, clone and sequence four different fragments covering about 72% of the genome of these virus isolates. The number of amplified isolates and useful sequence information varied according to each fragment, whereas an amplicon was obtained with diagnostic primers for 59 out of 60 isolates (98%). Phylogenetic analyses performed with obtained sequences and additional published sequences of 11 SCYLV isolates (GenBank accessions) showed that SCYLV isolates were distributed in different phylogenetic groups or belonged to single genotypes. The majority of isolates from Réunion Island were grouped in phylogenetic clusters that did not contain any isolate from other origins. The complete six ORFs (5612 bp) of five SCYLV isolates (two from Réunion Island, one from Brazil, one from China and one from Peru) were amplified, cloned and sequenced. The existence of at least three strains of SCYLV (strain BRA, strain PER and strain REU) was shown by the phylogenetic analysis of the sequence of these isolates and additional published sequences of three SCYLV isolates (GenBank accessions). The biological significance of these strains and the origin of the unique lineage of SCYLV in Réunion Island remain to be determined.

Last Modified: 10/22/2014
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