Technical Abstract: The in vitro binding of bile acids by spinach (spinacia oleracea), kale (brassica oleracea acephala), brussels sprouts (brassica oleracea gemmifera), broccoli (brassica oleracea italica), mustard greens (brassica juncea), peppers green (capsicum annuum), cabbage (brassica oleracea capitala) and collards (brassica oleracea acephala) was determined using a mixture of bile acids secreted in human bile at a duodenal physiological pH of 6.3. Six treatments and two blank incubations were conducted testing various fresh raw green vegetables on an equal dry matter basis. Considering cholestyramine (bile acid binding, cholesterol-lowering drug) as 100% bound, the relative in vitro bile acid binding on dry matter and total dietary fiber basis was for the spinach 9 and 34%, kale 8 and 64%, brussels sprouts 8 and 29%, broccoli 5 and 19%, mustard greens 4 and 12%, peppers green 3 and 9%, cabbage 2 and 8%, and collards 2 and 5%. Bile acid binding for spinach, kale and brussels sprouts was significantly higher than broccoli and mustard greens. For broccoli and mustard greens binding values were significantly higher those for cabbage, bell pepper and collards. These results point to the health promoting potential of spinach = kale = brussels sprouts > broccoli = mustard greens > cabbage = peppers green = collards as indicated by their bile acid binding on dry matter basis. Inclusion of spinach, kale and brussels sprouts in our daily diet as healthful dark green vegetables should be encouraged. Animal studies are planned to validate in vitro bile acid binding of vegetables observed herein to their healthful potential of atherosclerosis amelioration (lipid and lipoprotein lowering) and cancer prevention (excretion of toxic metabolites).