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ARS Home » Southeast Area » Fort Pierce, Florida » U.S. Horticultural Research Laboratory » Subtropical Insects and Horticulture Research » Research » Publications at this Location » Publication #182277
Title: DETERMINING THE ROLE OF ETHYLENE IN THE DEVELOPMENT OF TOMATO IRREGUALR RIPENING DISORDER USING MICROARRAY TECHNOLOGY AND RT-REAL TIME PCR

Author
item McKenzie, Cindy
item Shatters, Robert - Bob
item Albano, Joseph
item SINISTERRA, X - UNIV. OF FLORIDA
item POWELL, C - UNIV. OF FLORIDA

Submitted to: Entomological Society of America Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 10/1/2005
Publication Date: 11/6/2005
Citation: Mckenzie, C.L., Shatters, R.G., Albano, J.P., Sinisterra, X.H., Powell, C.A. 2005. Determining the role of ethylene in the development of tomato irregular ripening disorder using microarray technology and rt-real time pcr. Entomological Society of America Annual Meeting.

Interpretive Summary:

Technical Abstract: In order to determine the underlying causes of tomato irregular ripening disorder associated with whitefly feeding, microarray hybridization analysis followed by reverse transcription real time PCR validation were used to determine gene regulation in young and old leaf tissue, stems, flowers, roots, and fruits. Based on sequence similarity analysis we determined that selected genes were likely to be involved with ethylene production. Results will be presented and ethylene’s role in the development of tomato irregular ripening disorder will be discussed.