ARS | Publication request: EFFECT OF LEAF EXPLANTS ORIENTATION ON ADVENTITIOUS SHOOT REGENERATION OF PEAR

Submitted to: International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species
Publication Type: Abstract Only
Publication Acceptance Date: June 20, 2005
Publication Date: September 16, 2005
Citation: Bell, R.L. 2005. Effect of leaf explants orientation on adventitious shoot regeneration of pear. International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species. p. 15.

Technical Abstract: The literature on adventitious shoot regeneration is contradictory with respect to the effect of orientation of leaf explants with regard to the medium surface. Few studies have compared both adaxial and abaxial surfaces. The tendency of leaf explants to expand, curl, and lose complete contact with the medium is a secondary factor affecting regeneration efficiency. After four weeks of incubation on shoot induction medium, explants of young expanding leaves from in vitro shoot cultures with an adaxial surface in contact with the medium exhibited a small decrease in contact. Trimming the lamina to a rectangular piece of tissue had no effect. After an additional four weeks on auxinless shoot expression medium, trimmed explants with an abaxial surface in contact tended to curl, lift, and lose some contact with the medium. Trimming caused a significant decrease in regeneration, and contact of the abaxial surface with the media resulted in a 10% increase in regenerating untrimmed explants. There was a significant correlation between percent surface contact and the number of regenerated shoots only for untrimmed adaxial-contact explants.