Technical Abstract: This report describes an avian influenza A H5N2 virus in a red-lored Amazon parrot (Amazona autumnalis autumnalis) with clinical signs of diarrhea, melena and lethargy. This is the first report of H5N2 avian influenza A virus isolated from a psittacine bird. The virus was further characterized as a low pathogenic avian influenza strain and was closely related to a virus lineage that previously had only been reported in Mexico and Central America. This represents the first introduction of this virus lineage into the U.S., likely by illegal importation of psittacine birds. The avian influenza virus initially was detected by real-time RT-PCR (RRT-PCR) as part of a diagnostic evaluation that included Newcastle disease virus. Although detection of H5 avian influenza in birds in the U.S. typically results in euthanasia of the infected birds, an alternative strategy using strict quarantine measures and repeated diagnostic testing was used. The bird recovered from the initial clinical signs after four days and was released from quarantine nine weeks after intial presentation following two consecutive negative virus isolation and RRT-PCR results. Influenza A virus should be considered as a differential diagnosis for clinical signs of gastrointestinal disease in psittacine birds, especially in birds with a suspicious history of origin.