ARS | Publication request: MAPPING FOR COLD TOLERANCE IN TWO RECOMBINANT INBRED LINE POPULATIONS

Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: April 1, 2005
Publication Date: July 1, 2005
Citation: Reddy, L., Song, J.H., Cregan, P.B., Costa, J., Garland Campbell, K.A. 2005. Mapping for cold tolerance in two recombinant inbred line populations. Agronomy Abstracts. http://crops.confex.com/crops/2005am/techprogram/P5379.HTM

Technical Abstract: Wheat is the third major grain crop in the U.S after corn and soybeans. Winter wheat contributes about 70% of the total wheat produced in the country. Winter wheat production is affected by cold and freezing injury depending on the type of winters the farmers face. We are using SSR markers to map QTLs for cold tolerance and vernalization, and to identify markers that can be used for marker assisted selection for winter hardiness. Two RIL populations Norstar/Centurk78 and Karl/Z0031 are being used to map QTLs for cold tolerance. Both gwm and barc primers are being used for the populations. Initial screening has been done using SSR primers of the gwm and barc series, 119 primers are polymorphic for the Norstar/Centurk78 population and 137 polymorphic primers are identified for the Karl/Z0031 population. These two populations have been replicated and tested for their cold hardiness in the greenhouse, we used artificial freeze test and their LT50 values have been determined. The LT50 values were –12.43oC for Z0031, –18.19oC for Karl, -18.62oC for Norstar and -16.01oC for Centurk78 respectively. Currently the populations are being genotyped for mapping cold tolerance.