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United States Department of Agriculture

Agricultural Research Service

Title: Quantification of Ptgs in Nicotiana Species Using Different Reporter Genes and Viral Suppressor Proteins

item Cazzonelli, Christopher
item Velten, Jeffrey

Submitted to: Keystone Symposia
Publication Type: Proceedings
Publication Acceptance Date: September 16, 2004
Publication Date: January 8, 2005
Citation: Cazzonelli, C.I., Velten, J.P. 2005. Quantification of ptgs in nicotiana species using different reporter genes and viral suppressor proteins [abstract]. Keystone Symposia.

Technical Abstract: In plants, posttranscriptional gene silencing (PTGS) is a highly conserved nucleotide sequence-specific RNA turnover mechanism that can recognize foreign RNA molecules and lead to the double stranded RNA-dependent silencing of transgenes. The mechanism(s) by which plants detect aberrant or foreign gene expression and trigger post-transcriptional gene silencing remains to be elucidated. Recent evidence supports the hypothesis of an aberrant model, rather then a threshold effect. By use of an Agrobacteria-mediated in vivo transient assay system developed for tabacco (Nicotiana tobacum and benthamiana) we have investigated the effects of the p1 and HcPro suppressors of PTGS, on the two luciferase reporter genes, FiLUC (Firefly, Phontinus pyralis) and RiLUC (sea pansy, Renilla reniformis). We have observed differences in the suppressed luciferase activities of the two reporter genes. Our data supports the idea that the sequence identity/secondary structure of the reporter gene transcript is a significant determinant of how the foreign transcript is perceived and PTGS is triggered and or maintained.

Last Modified: 4/20/2015
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