ARS | Publication request: ISOLATION AND CHARACTERIZATION OF 15 MICROSATELLITE LOCI FROM MANGO (MANGIFERA INDIA L.) AND CROSS-SPECIES AMPLIFICATION IN CLOSELY RELATED TAXA

Submitted to: Molecular Ecology Notes
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 1, 2005
Publication Date: June 5, 2005
Citation: Schnell II, R.J., Olano, C.T., Quintanilla, W.E., Meerow, A.W. Isolation and characterization of 15 microsatellite loci from mango (Mangifera India L.) and cross-species amplification in closely related taxa. Molecular Ecology Notes. 5:625-627.

Interpretive Summary: The estimation of genetic diversity in our germplasm collections is a major goal of the National Plant Germplasm System. One of the best methods to accomplish this is with the use of molecular markers. The best molecular markers to use are microsatellite markers due to their co-dominant nature. We report on the development of 15 new microsatellite markers for mango. These markers can now be used for cultivar identification and genetic diversity studies.

Technical Abstract: We report here on the development and characterization of 15 microsatellite loci isolated from Mangifera indica L. These markers were evaluated using 59 Florida cultivars and four related species from the USDA germplasm collection for mango. Two loci were monomorphic and thirteen polymorphic, with two to seven alleles per locus. Four loci departed significantly from Hardy-Weinberg Equilibrium and have significant heterozygote deficiency. Nine loci exhibited significant linkage disequilibrium. Cross-species amplification was successful in the four related species. These loci are being used to investigate patterns of genetic variation within M. indica and between closely related species.