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Title: SELECTION OF LACTOBACILLUS SPP FROM POULTRY INTESTINE PROVIDING NEW PROBIOTIC STRAINS

Author
item DENISOV, A - RES CTR FOR TOXICOLOGY
item MALICK, E - ST RES CTR RUSSIA
item MALICK, N - ST RES CTR RUSSIA
item Stern, Norman
item RYBAKOVA, O - RES CTR FOR TOXICOLOGY
item DOROVATOVSKAYA, J - RES CTR FOR TOXICOLOGY
item GNEDENKOV, P - RES CTR FOR TOXICOLOGY
item LOSEVA, J - RES CTR FOR TOXICOLOGY

Submitted to: Symposium on Lactic Acid Bacteria Genetics Metabolism and Applications
Publication Type: Abstract Only
Publication Acceptance Date: 3/29/2005
Publication Date: 5/8/2005
Citation: Denisov, A.A., Malick, E.V., Malick, N.I., Stern, N.J., Rybakova, O.M., Dorovatovskaya, J.S., Gnedenkov, P.L., Loseva, J.G. 2005. Selection of lactobacillus spp from poultry intestine providing new probiotic strains. Symposium on Lactic Acid Bacteria Genetics Metabolism and Applications.

Interpretive Summary:

Technical Abstract: We isolated strains of Lactobacillus spp. from 24-day old chicken intestines. Our purpose was to select the most desirable isolates enabling the creation of effective probiotics. We hypothesized that the primary selection criteria for effective probiotics included: 1) the degree of strain colonization, 2) the adhesive capability of the lactobacilli isolates, and 3) the antagonistic activity and growth properties of the isolates. To assess these criteria, we enriched the poultry intestinal samples, isolated pure cultures, and on 'RS and Rogosa media determined the morphological, cultural, fermentative and biochemical properties of the lactobacilli isolates . Adhesive properties were determined using formalinized human erythrocytes from group 0 (I), Rh-positive blood. Antagonistic activity was evaluated by inoculating wells among agar cultures of E. coli, Salmonella, Shigella, Staphylococcus (and other microorganisms) to assess zones of inhibition created by the indicator cultures (the lactobacilli test-cultures). As a result, 83 Lactobacillus spp. isolates having different adhesive and antagonistic properties were obtained. All isolates were conditionally divided into 4 groups by degree of adhesive activity. Only a few isolates having high adhesive properties also possessed a wide spectrum of antagonistic activity and ability to rapidly grow on nutrient media. Based upon the entire study of characteristics, five lactobacilli isolates were considered as primary probiotic strain candidates. Cultural-and-morphological, biochemical properties and phenotyping of these isolates were completed. Identification of the isolates using API-test strips showed that they belonged to different species of Lactobacillus.