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Title: FAILURE OF SHORT TERM FEED RESTRICTION TO EFFECT LEPTIN SECRETION AND SUBCUTANEOUS ADIPOSE TISSUE EXPRESSION OF LEPTIN RECEPTOR (OB-R) IN THE PREPUBERAL GILT

Author
item HART, HEATHER - UNIV OF GEORGIA
item AZAIN, MICHAEL - UNIV OF GEORGIA
item Hausman, Gary
item REEVES, DAVID - UNIV OF GEORGIA
item Barb, Claude

Submitted to: Journal of Animal Science Supplement
Publication Type: Abstract Only
Publication Acceptance Date: 2/27/2005
Publication Date: 7/20/2005
Citation: Hart, H.A., Azain, M.J., Hausman, G.J., Reeves, D.E., Barb, C.R. 2005. Failure of short term feed restriction to effect leptin secretion and subcutaneous adipose tissue expression of leptin receptor (ob-r) in the prepuberal gilt [abstract]. Journal of Animal Science Supplement. 83(1):167.

Interpretive Summary:

Technical Abstract: Ovariectomized prepuberal gilts averaging 164 days of age and 79.2 ± 3.8 kg body weight (BW) were either fed to appetite (FA; n = 6) or feed restricted (RST; 40% of FA diet; n = 6) for 7 seven days to determine the effects of feed RST on serum leptin concentrations, metabolism, leptin, Ob-r and transcription factor expression in subcutaneous back fat (BF). On day 8, blood samples were collected every 15 min for 8 h. Serum concentrations of glucose, T3, T4, NEFA, insulin, and leptin were determined. Real-time PCR was performed on mRNA extracted from subcutaneous adipose tissue collected on day 0 (start RST) and day 9. FA gilts gained (P<.0001) more BW (8.3 ± .6 kg) than RST (-2.5 ± .6 kg) gilts, however BF thickness did not change. A treatment x time interaction (P<.009) was detected for serum glucose concentrations. Serum insulin (P<.07), T3 (P<.08), and T4 (P<.04) concentrations were reduced and NEFA levels (P<.05) were greater during h 1, 6, 7 and 8 in RST gilts compared to FA animals demonstrating a metabolic response to RST. Serum leptin concentrations, leptin pulse amplitude, and leptin pulse frequency were not effected by RST. RST failed to effect subcutaneous BF leptin, Ob-r, AFABP (adipocyte fatty acid binding protein), C/EBP-alpha (CCAAT/enhancer binding protein alpha), or PPAR- gamma 2 (peroxisome proliferator activated receptor gamma 2) mRNA expression compared to FA gilts. These results may in part be related to the failure of RST of this duration to influence subcutaneous BF. Thus, the leptin response to RST may require energy levels and (or) BF reduction reaching a putative inhibitory threshold.