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Title: EVALUATION OF RESPONSE OF A NEW EXPERIMENTAL LINE OF CHICKENS (LINE 0-1) TO AVIAN LEUKOSIS VIRUS INFECTION

Author
item Mays, Jody
item Hunt, Henry
item PANDIRI, ARUN - MICHIGAN STATE UNIV
item Bacon, Larry
item Fadly, Aly

Submitted to: American Veterinary Medical Association Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/16/2005
Publication Date: 7/16/2005
Citation: Mays, J.K., Hunt, H.D., Pandiri, A., Bacon, L.D., Fadly, A.M. 2005. Evaluation of response of a new experimental line of chickens (line 0-1) to avian leukosis virus infection [abstract]. American Veterinary Medical Association. p. 58.

Interpretive Summary:

Technical Abstract: We previously reported the development of a new chicken line (line 0-1) by mating the original ADOL line 0 to a commercial layer lacking endogenous viral genes (Bacon et al. Avian Pathol. 2004: 33:233-243). In the present study, we compared the response of chickens from line 0-1 with chickens of the original line 0 following infection with strain RAV-1 of subgroup A avian leukosis virus (ALV-A) and strain ADOL Hc-1 of ALV-J at hatch. At 5, 8, 12, and 16 weeks of age chickens were tested for viremia and neutralizing antibody. The cytotoxic T-cell (CTL) response against ALV-J was also measured in both lines. No significant differences in viremia and antibody responses between line 0-1 and line 0 chickens were noted following infection with ADOL Hc-1. However, line 0-1 and line 0 chickens differed in their RAV-1 antibody response. At 5 weeks of age, 11/13 (85%) of line 0-1 chickens produced neutralizing antibody, compared with 4/12 (33%) of line 0 chickens. By 12 weeks of age, the majority of line 0-1 and line 0 chickens had cleared the virus and were producing neutralizing antibody. Also, no significant differences were noted in tumor response between line 0-1 and line 0 chickens following RAV-1 infection. In addition, the CTL response was comparable between the two lines. These characteristics indicate that line 0-1 is suitable for use in research and diagnosis of ALV.