ARS | Publication request: IDENTIFICATION OF FUNGAL AND YEAST ISOLATES BY ANALYSIS OF NUCLEAR LARGE SUBUNIT(26S) RIBOSOMAL DNA SEQUENCES

Submitted to: Aflatoxin Workshop
Publication Type: Abstract Only
Publication Acceptance Date: October 25, 2004
Publication Date: N/A

Technical Abstract: Yeast and fungal strains were isolated from pistachio and almond orchards as single colonies. Yeast cells and fungal mycelia were harvested from cultures medium by centrifugation and DNA were extracted for PCR reaction. A pair of universal primers, NL1 and NL4 were used to generate the fragment of D1/D2 region in the 26S ribosomal subunit. The nucleotide sequences of each fragment were determined using the ABI Prism BigDyeTM Terminator Cycle Sequencing Ready Reaction Sequencer Kit and ABI Prism 3100 Genetic Analyzer. The sequences were Blast searched using GenBank database and DNA data libraries (DDBJ/EMBL). Yeast species identified are Pichia anomala, Rhodotorula mucilaginosa, Pichia fermantans, Candida zeylanoides, Issachenkia orientalis, Pichia guillermondi. Fungal species identified are Aspergillus flavus, Aspergillus carbonarius, Aspergillus phoenicis, Cladosporium cladosproriodes, Alternaria alternata.