|Pereyra, Rt - CISESE|
|Saillant, E - TEXAS A&M UNIVERSITY|
|Pruett, Cl - TEXAS A&M UNIVERSITY|
|Rocha-Olivares, A - CISESE|
|Gold, Jr - TEXAS A&M UNIVERSITY|
Submitted to: Molecular Ecology Notes
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: August 10, 2004
Publication Date: November 10, 2004
Citation: Pereyra, R., Saillant, E., Pruett, C., Rexroad III, C.E., Rocha-Olivares, A., Gold, J. 2004. Characterization of polymorphic microsatellites in the pacific sardine sardinops sagax sagax (clupeidae). Molecular Ecology Notes 4(4):739-41. Interpretive Summary: Pacific sardines are a vital economic resource in the northeastern Pacific Ocean, but very little information is available for characterizing the genetic makeup of this species. To this end, we developed eleven DNA markers from the Pacific sardine Sardinops sagax sagax. Observation of the genetic variation of these markers in a sample of 30 individuals suggests that they will be useful for broader studies aimed at determining genetic variation and population structures of this species.
Technical Abstract: We isolated eleven microsatellites from the Pacific sardine Sardinops sagax sagax. The number of alleles and heterozygosity among 30 individuals from a single population ranged from 4 to 24 and from 0.606 to 0.959, respectively. Pacific sardines are a vital economic resource in the northeastern Pacific Ocean, but insufficiently polymorphic loci have limited inferences about its stock structure and genetic variation. The level of variability of these new markers will prove useful in testing hypotheses on the stock-structure and long-term genetic integrity of the species.