Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: September 1, 2004
Publication Date: November 30, 2004
Citation: Ow, D.W. 2004. Gene stacking through site-specific integration. In: G.H. Liang, D.Z. Skinner, editors. Genetically Modified Crops, Their Development, Uses and Risks. Haworth Press, Inc. pp. 71-100. Technical Abstract: The exact placement of foreign DNA into the plant genome produces transgenes with greater structural fidelity and faithful expression. Recombinase-mediated site-specific integration has been reported for several plant species, including rice and maize. The next challenge will be to develop a practical strategy to stack DNA. Being able to append new DNA sequentially to a target site permits the continual use of a previously characterized chromosome location, which justifies the initial investment costs in identifying favorable chromosome targets. Stacking transgenic traits at a limited number of target sites is also preferable to scattering transgenes all over the genome, as the clustering of transgenes expedites the introgression of bundled traits to elite cultivars. This chapter reviews the current status of DNA site-specific integration in plants, and outlines methods for stacking DNA onto a chromosomal target.