Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: November 29, 2004
Publication Date: February 7, 2005
Citation: Weiland, J.J., Larson, R.L., Lewellen, R.T. 2005. Progress on resistance gene tagging and the development of functional genomics tools in sugarbeet. Plant & Animal Genomes XIII Conference. W336. Available: http://www.intl-pag.org/13/abstracts/PAG13_W336.html Technical Abstract: The availability of advanced segregating population of sugarbeet (Beta vulgaris L.) is being exploited to obtain molecular genetic markers linked to these traits. In recent years, robust markers for the loci conditioning resistance to root knot nematode and beet mosaic virus have been produced, whereas such markers for resistance to powdery mildew conferred by the dominant Pm gene have been less reliable. An update will be presented on Pm tagging as well as on future projects aimed at tagging loci for resistance to sugarbeet cyst nematode. In parallel work, vector systems for the delivery of double-stranded RNA (dsRNA), thereby generating RNA interference, are being developed both for the modification of sugarbeet gene expression and for the high-throughput determination of optimal sequences for sugarbeet protection from plant viruses. One vector chosed for dsRNA delivery has been barley stripe mosaic virus, which causes a local infection on inoculated leaves of sugarbeet. Using this vector, it was determined that a higher degree of foliar protection from challenge-inoculation with beet necrotic yellow vein virus was achieved, as determined by disease reduction and ELISA assays, when targeting the untranslated regions RNA1 as compared to constructs containing RNA1 coding region guide sequences.