|Schuschke, Dale - UNIV OF LOUISVILLE|
|Gordon, Sharon - UNIV OF LOUISVILLE|
|Lentsch, Alex - UNIV OF CINCINNATI|
Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: December 1, 2004
Publication Date: March 7, 2005
Citation: Schuschke, D.A., Gordon, S.A., Saari, J.T., Lentsch, A.B. 2005. Impaired deformability of copper-deficient neutrophils [abstract]. The Federation of American Societies for Experimental Biology Journal. 19(5):A1485. Technical Abstract: We have previously shown that dietary copper deficiency promotes neutrophil accumulation in the lung microvasculature. The current study was designed to determine whether copper deficiency promotes neutrophil chemoattraction within the lung vasculature or if it alters the mechanical properties of the neutrophil thus restricting passage through the microvessels. Sprague-Dawley rats were fed purified diets which were either copper-adequate (CuA; 6.3'g Cu/g diet) or copper-deficient (CuD; 0.3 'g Cu/g diet) for 4 weeks. To assess neutrophil chemoattraction, bronchoalveolar lavage fluid was assayed for the neutrophil chemokine macrophage inflammatory protein-2 (MIP-2) by ELISA. Neutrophil deformability was determined by measuring the pressure required to pass isolated neutrophils through a 5 'm polycarbonate filter. The MIP-2 concentration was not significantly different between the dietary groups (CuA 435.4 ± 11.9 vs CuD 425.6 ± 14.8 pg/ml) but more pressure was needed to push CuD neutrophils through the filter compared to controls (CuA 0.150 ± 0.032 vs CuD 0.284 ± 0.037 mmHg/min). These results suggest that dietary copper deficiency reduces the deformability of neutrophils. Since most neutrophils must change their shape during passage from arterioles to venules in lungs, we propose that CuD neutrophils accumulate in the lung because they are more resistant to shape change. Supported by NIH DK055030.