|French, Richard - UNIV. CONNECTICUT|
Submitted to: Meeting Abstract
Publication Type: Proceedings
Publication Acceptance Date: September 30, 2004
Publication Date: November 14, 2004
Citation: Risatti, G.R., Kutish, G.F., Lu, Z., Holinka, L.G., French, R.A., Tulman, E., Rock, D.L., Borca, M.V. 2004. The E2 Glycoprotein of Classical Swine Fever Virus is a Virulence Determinant in Swine. Conference of Research Worksers in Animal Disese Meeting. P. 196. Technical Abstract: E2 is one of the three envelope glycoproteins of Classical Swine Fever Virus (CSFV). Although E2 has been described as mediating virus attachment to target cells and is responsible for inducing a protective immune response, its role in viral pathogenesis remains unknown. To examine this, a chimera of the highly pathogenic CSFV isolate Brescia and the attenuated vaccine strain CS were constructed from their respective infectious clones and evaluated for virulence in pigs. The chimeric virus 319.1 (319.1v), containing the E2 glycoprotein of CS in the Brescia background, exhibited unaltered growth characteristics on primary porcine macrophage cell cultures, although a smaller plaque phenotype was observed on SK6 cell cultures. Notably, 319.1v was markedly attenuated in pigs exhibiting significantly decreased virus replication in tonsils, a transient viremia, limited generalization of infection and decreased virus shedding. These results indicate a highly significant role for the E2 glycoprotein in pig virulence.