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United States Department of Agriculture

Agricultural Research Service

Title: Differential Expression of Nat1 Translational Repressor During Development of Bovine Intramuscular Adipocytes

Authors
item Childs, Kirby - OKLAHOMA STATE UNIVERSITY
item Goad, David - OKLAHOMA STATE UNIVERSITY
item Allan, Mark
item Pomp, Daniel - UNIV NEBRASKA, LINCOLN
item Krehbiel, Clinton - OKLAHOMA STATE UNIVERSITY
item Geisert, Rodney - OKLAHOMA STATE UNIVERSITY
item Morgan, J - OKLAHOMA STATE UNIVERSITY
item Malayer, Jerry - OKLAHOMA STATE UNIVERSITY

Submitted to: Physiological Genomics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: May 30, 2002
Publication Date: August 1, 2002
Citation: Childs, K.D., Goad, D.W., Allan, M.F., Pomp, D., Krehbiel, C., Geisert, R.D., Morgan, J.B., Malayer, J.R. 2002. Differential expression of NAT1 translational repressor during development of bovine intramuscular adipocytes. Physiological Genomics. 10(2):49-56.

Interpretive Summary: This study was undertaken to test for differential gene expression in intramuscular adipocytes during fat deposition of feedlot steers. Angus x Hereford steers (n = 50) were fed a high-energy concentrate ration ad libitum for 20 (n = 5), 86 (n = 15), 121 (n = 15), and 146 days (n = 15) to obtain various degrees of intramuscular adipocyte development. Carcass traits were significantly different between the groups. Intramuscular adipose tissue was excised from the ribeye and snap frozen in liquid nitrogen. Samples of total gene message representing each group were analyzed. Putative differences among treatment groups were sequenced, and subjected to BLAST database search (NCBI). One was of particular interest, the translational repressor NAT1 (novel APOBEC-1 target-1). NAT1 gene message was quantified in individual animals to confirm differential expression among treatment groups. Results indicate that NAT1 message is more abundant in intramuscular adipocytes of younger/leaner animals.

Technical Abstract: This study was undertaken to test for differential gene expression in intramuscular adipocytes during fat deposition of feedlot steers. Angus x Hereford steers (n = 50) were fed a high-energy concentrate ration ad libitum for 20 (n = 5), 86 (n = 15), 121 (n = 15), and 46 days (n = 15) to obtain various degrees of intramuscular adipocyte development. Carcass traits were significantly different (P < 0.05) between the groups. Intramuscular adipose tissue was excised from the longissimus dorsi and snap frozen in liquid nitrogen. Pooled samples of total RNA representing each group were analyzed by differential-display polymerase chain reaction using 200 primer combinations comprising 20 arbitrary (5') and 10 anchor (3') oligonucleotides. Bands (n = 70) representing putative differences among treatment groups were excised, sequenced, and subjected to BLAST homology search. From these, 40 contained significant homology to known genes. One was of particular interest, the translational repressor NAT1 (novel APOBEC-1 target-1). NAT1 mRNA was quantified in individual animals to confirm differential expression among treatment groups. Results indicate that NAT1 message is more abundant (P < 0.05) in intramuscular adipocytes of younger/leaner animals.

Last Modified: 9/2/2014
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