|Ma, Xuefeng - UNIV OF MISSOURI-COLUMBIA|
Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: August 6, 2003
Publication Date: November 1, 2004
Citation: Ma, X., Gustafson, J.P. 2004. Genome variation in allopolyploid triticale [abstract]. ASA-CSSA-SSSA Annual Meeting Abstracts. Poster Board #1368. Technical Abstract: Genome variation in allopolyploid triticale was investigated using both AFLP and RFLP analyses. The restriction enzymes used for AFLP analyses were selected to primarily amplify repetitive and low-copy sequences with two sets of primers, EcoRI/MseI and PstI/MseI, respectively. To estimate the amount of variation occurred in expressed sequences, Southern hybridizations were performed with EST clones. The results showed that the genomic sequences in triticale involved a great degree of variation including both repetitive and low-copy sequences, and coding and non-coding sequences. The amount of variation in repetitive sequences was higher than that in low-copy sequences; and the variation in non-coding sequences was much higher than that in coding sequences. In all cases, the frequency of parental band loss was much higher than the frequency of gaining novel bands, suggesting that sequence elimination might be a major force causing genome variation in triticale. The data also showed that the wheat genomes were relatively highly conserved in triticales, especially in octoploid triticales, whereas the rye genome consistently demonstrated a very high level of genomic sequence variation (72% of repetitive and 68% of low-copy sequences) regardless of the triticale ploidy levels or primers used. The data also suggested that concerted evolution might occur in the genomic sequences of triticale.