SECRETED AND SIGNALING GENES AND PROTEINS IN FETAL AND NEONATAL PIG ADIPOSE TISSUE AND STROMAL-VASCULAR CELLS.

Authors: Hausman, Gary; Poulos, Sylvia; Richardson, Richard; Barb, Claude; ANDACHT, TRACY - UNIVERSITY OF GEORGIA; MYNATT, RANDALL - PENNINGTON BIOMED. RES. C; PECOT, HEATHER - PENNINGTON BIOMED. RES.C.; CRANDALL, DAVID - WYETH RESEARCH LABS; HREHA, AMY - WYETH RESEARCH LABS

Submitted to: Journal of Animal Science
Publication Type: Abstract Only
Publication Acceptance Date: 4/15/2004
Publication Date: 6/1/2004
Citation: Hausman, G.J., Poulos, S.P., Richardson, R.L., Barb, C.R., Andacht, T., Mynatt, R., Pecot, H., Crandall, D., Hreha, A. Secreted and signaling genes and proteins in fetal and neonatal pig adipose tissue and stromal-vascular cells.. Journal of Animal Science. 2004. v. 82. suppl. 1. p. 200.

Interpretive Summary:

Technical Abstract: Although microarray studies indicate unique and unexpected genes in human and rodent adipose tissue similar studies of meat animal adipose tissue have not been reported. Total RNA was isolated from 90 day fetal stromal-vascular (S-V) cell cultures (n=4; 2 arrays ,2 cultures/ array) and subcutaneous adipose tissue from two105 day-old fetuses and neonatal pigs. Dye labeled cDNA probes were hybridized to custom microarrays (70 mer oligonucleotides) representing 600 pig genes involved in growth and reproduction. Each of the four adipose tissue arrays represented RNA from a fetus and a pig with duplicate arrays / fetus and pig. Relative intensities of 25 irrelevant or reproductive oriented genes were averaged for both S-V cell culture arrays (150 ± 20) and all adipose tissue arrays (200 ± 40) and used to represent basal gene expression. A total of 391 and 340 genes were expressed 10 fold and 200 and 160 genes expressed 40 fold over basal in S-V cultures and adipose tissue, respectively. Relative intensities in adipose tissue arrays (n = 4) included 20,360± 2700 for IGFBP-5, 19,800± 2,800 for relaxin, 7,360 ± 1,260 for leptin and 2,390± 355 for APO-A1. Collectively, 21 secreted protein genes and 40 signaling protein genes were expressed 40 fold in S-V cultures and adipose tissue. Additionally, the agouti gene was detected by RT-PCR in pig S-V cultures and adipose tissue. Proteomic analysis of adipose tissue and S-V culture conditioned media and cells identified several secreted and signaling proteins including APO-A1, APO-E, relaxin, IGFBP-5 and nitric oxide synthase. Another of the secreted proteins, PAI-1, was identified by ELISA in S-V culture media. These studies demonstrate for the first time the expression of several major secreted and signaling proteins in pig adipose tissue which may influence local and central metabolism and growth.