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ARS Home » Midwest Area » Columbus, Ohio » Soil Drainage Research » Research » Publications at this Location » Publication #167467
Title: TRANSCRIPTIONAL PROFILING OF HYPOXIC STRESS RESPONSE IN ARABIDOPSIS

Author
item LIU, FENGLONG - TIGR
item Vantoai, Tara
item MOY, LINDA - TIGR
item BOCK, GEOFFREY - TIGR
item LINFORD, LARA - TIGR
item QUACKENBUSH, JOHN - TIGR

Submitted to: Mid Atlantic Plant Molecular Biology Society Conference
Publication Type: Abstract Only
Publication Acceptance Date: 4/20/2004
Publication Date: 8/19/2004
Citation: Liu, F., Vantoai, T.T., Moy, L., Bock, G., Linford, L., Quackenbush, J. 2004. Transcriptional profiling of hypoxic stress response in arabidopsis. Mid Atlantic Plant Molecular Biology Society Conference. p. 18. .

Interpretive Summary:

Technical Abstract: Plants have evolved adaptation mechanisms to sense oxygen deficiency in their environment and make coordinated physiological and structural adjustments to enhance their hypoxic tolerance. To gain insight into how plants respond to low oxygen stress, gene expression profiling was carried out at 9 time points over 24 hours, in wild-type and flooding-tolerant PSAG12:ipt Arabidopsis plants under normoxic and hypoxic conditions, using whole-genome DNA amplicon microarrays. Transcript level of genes involved in glycolysis and fermentation pathways, ethylene synthesis and perception, calcium signaling, nitrogen utilization, trehalose metabolism, and alkaloid synthesis was significantly altered in response to oxygen limitation. Analysis based on gene ontology (GO) terms suggested a significant down-regulation of genes whose functions are associated with cell walls, nucleosome structures, water channels and ion transporters, and a significant up-regulation of genes involved in transcriptional regulation and auxin responses under conditions of oxygen shortage. Promoter analysis on a cluster of up-regulated genes revealed a significant over-representation of the AtMYB2 binding motif (GT-motif), a sugar response element (SURE)-like motif and a G-box-related sequence, and also identified several putative anaerobic response elements. Finally, the real time PCR using 29 selected genes independently verified the microarray results. The study represents one of the most extensive studies conducted to date investigating the hypoxia-perturbed transcriptional networks in plants.