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United States Department of Agriculture

Agricultural Research Service

Title: Development and Characterization of Rice Mutant Populations for Functional Genomics of Host-Parasite Interactions

item Rutger, J
item Singh, P - UA RREC
item Martin, R - OAK RIDGE NATL LAB

Submitted to: American Phytopathological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: June 30, 2004
Publication Date: July 30, 2004
Citation: Jia, Y., Rutger, J.N., Wang, Z., Singh, P., Martin, R., Pinson, S.R. 2004. Development and characterization of rice mutant populations for functional genomics of host-parasite interactions. American Phytopathological Society Annual Meeting. Phytopathology. 94(6):47.

Technical Abstract: Understanding molecular mechanisms of R gene mediated defense responses should facilitate the development of both conventional and novel strategies for disease control. To dissect R gene-mediated signal transduction pathways the blast resistant US rice cultivar Katy was mutagenized by ethyl methane sulfonate (EMS) and fast neutrons. For EMS, seeds were soaked in 0.4%, 0.8% and 1.2 % EMS solutions for 6 hours and a total of 7,199 M1 mutant lines was recovered. For fast neutron, seven dosages of fast neutron were applied and a total of 15,400 M1 mutant lines was recovered from treatments with 7.7 Gray, 26.3 Gray and 49.4 Gray. Preliminary analysis of M2 seedlings revealed defects in chlorophyll synthesis in approximately 4% of M1 derived lines. Katy containing the Pi-ta gene prevents the infection of Magnaporthe grisea races in a gene for gene manner. M. grisea races containing the avirulence gene AVR-Pita are being used to identify blast susceptible mutants. A total of 42 blast susceptible mutants and 7 lesion mimic mutants were identified. Progress on characterization of these mutants will be reported.

Last Modified: 4/22/2015
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