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Title: MAIZE CHROMOSOME ADDITIONS AND RADIATION HYBRIDS IN OAT AND THEIR USE IN DISSECTING THE MAIZE GENOME

Author
item Rines, Howard
item PHILLIPS, R - UNIVERSITY OF MINNESOTA
item KYNAST, R - UNIVERSITY OF MINNESOTA
item OKAGAKI, R - UNIVERSITY OF MINNESOTA
item ODLAND, W - UNIVERSITY OF MINNESOTA
item STEC, A - UNIVERSITY OF MINNESOTA
item JACOBS, M - UNIVERSITY OF MINNESOTA
item GRANATH, S - UNIVERSITY OF MINNESOTA

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 9/30/2004
Publication Date: 9/1/2005
Citation: Rines, H.W., Phillips, R.L., Kynast, R.G., Okagaki, R.J., Odland, W.E., Stec, A.O., Jacobs, M.E., Granath, S.R. 2005. Maize chromosome additions and radiation hybrids in oat and their use in dissecting the maize genome. In: Tuberosa, R., Phillips, R.L., Gale, M., editors. In the Wake of the Double Helix: From the Green Revolution to the Gene Revolution. Proceedings of an International Congress. University of Bologna, Italy. May 27-31, 2003. Bologna, Italy: Avenue Media. p. 427-442.

Interpretive Summary:

Technical Abstract: Plant recovery from wide-cross sexual hybridization between members of two subfamilies of grasses, as initially described in the production of haploid wheat plants in wheat x maize crosses, can also be obtained in oat x maize crosses. In wheat x maize crosses haploid wheat plants are obtained as a result of complete elimination of the maize chromosomes prior to embryo rescue. In contrast, about one-third of the plants from oat x maize crosses contain one or more maize chromosomes in addition to a complete haploid complement of 21 oat chromosomes. Over the past few years all ten maize chromosomes have been recovered as single chromosome additions to a haploid oat complement, and fertile disomic addition lines in oat have been obtained for maize chromosomes 1 through 9 as well as a ditelosomic from maize chromosome 10 short arm. Polymerase chain reaction (PCR) assays of DNA extracts for the maize-specific Grande-1 dispersed repeated element are used to detect plants with maize chromatin. Assays with maize chromosome-specific markers are used to identify which maize chromosome(s) is present, and genomic in situ hybridization analysis of root-tip cells is performed to visualize the number of maize chromosomes present. Plant phenotypes, transmission characteristics of addition chromosomes, and potential uses of the oat-maize addition lines are described here. We also emphasize the value of these strains for mapping duplicated maize sequences and gene families. Gamma irradiation of oat-maize addition lines is used to produce radiation hybrid lines with maize chromosomal segment deletions and translocations. Finally, we describe the use of radiation hybrids having overlapping deficiencies to enable physical mapping of maize sequences to maize chromosome segments.