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United States Department of Agriculture

Agricultural Research Service

Title: Protocol for DNA Extraction from Watermelon Leaves for Ssr Marker Studies

Authors
item Gusmini, Gabriele - NCSU
item Wehner, Todd - NCSU
item Joobeur, Tarek - NCSU
item Dean, Ralph - NCSU
item Levi, Amnon

Submitted to: Cucurbit Genetics Cooperative Report
Publication Type: Abstract Only
Publication Acceptance Date: August 15, 2004
Publication Date: N/A

Technical Abstract: Due to the high amounts of phenolic compounds in watermelon leaves, it is difficult to isolate high quality DNA from their tissue employing isolation procedures commonly used with various plant species. In this study, a procedure for isolating high-quality DNA from a few watermelon leaf samples was modified for isolating DNA from a large number of leaf samples (24 samples per run; 500 mg of ground fresh leaf tissue). The modified DNA isolation procedure is suitable for fast analysis of watermelon genome using simple sequence repeat (SSR), randomly amplified polymorphic DNA (RAPD), or sequenced characterized amplified regions (SCAR) markers.

Last Modified: 10/1/2014
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